Extra Virgin Cod Liver Oil Nutritional Analysis

EVCLO - Nutritional Analysis

A wide range of nutritional tests have been performed on Rosita EVCLO. The “Nutritional Analysis” presented represent only a small fraction of this tests which include vitamins, and specialist chemistry testing.

evclo raw fresh2

1. Nutrient Results

2. Purity

3. Oxidation byproducts

4. Hydrolytic Rancitiy Measures

5. Microbiological Testing

6. Nutrition Facts

7. Shelf-Life Study Results

1. Nutrient Results

The concentration of fat-soluble nutrients depends on several biological factors, including the size of the fish and the amount of oil contained in the liver. There are also seasonal variations related to the spawning cycle of the fish, climatic conditions, plankton, and other conditions affecting the codfish. Because Rosita EVCLO is a completely unrefined & unprocessed wholefood, the concentration of nutrients will ALWAYS vary from batch to batch.

The Nutrient Results (fat-soluble vitamins, sterols & fatty acids) shown below represent typical values and ranges which have been obtained from analytical testing at the following institutes:

NIFES (National Institute of Nutrition and Seafood Research )[20/12/2010]

ALS Food & Pharmaceutical (United Kingdom) [10/10/2012]

Eurofins Food Testing [19/02/2013]

Nutrition Analytical Service (NAS), Institute of Aquaculture, University of Stirling, Scotland, United Kingdom [15/11/2013]. The head of NAS, Professor Gordon Bell, has over 30 years of experience in fish nutrition and lipid and nutrition related analysis and has published more than 184 peer-reviewed papers on fish nutrition, fatty acids, lipid soluble nutrients and antioxidants. The Chief Analyst, James Dick, provides over 25 years of experience in lipid methodology and is responsible for Quality Control within the Nutrition Group, the development of chromatographic techniques and is trained in all aspects of complex lipid analysis including HPLC, GC, GC/MS and GC/MS/MS.

Extra virgin oil was also tested [as far back as: 15.07.2011] by the producer at ECO-Marine using Nofima (the Norwegian Institute of Food, Fisheries and Aquaculture Research) BioLab which specialises in marine raw materials and products. Project no (C3937).

VITAMIN E (ALPHA-TOCOPHEROL) ANALYSIS

SAMPLE ID	mg/teaspoon
Wild & Raw EVCLO	3.7-10

Above values calculated from analyses performed at a number of European testing institutes

(named above), and each testing institute analyses has been performed in duplicate.

VITAMIN A (RETINOL) ANALYSIS

SAMPLE ID	iu/teaspoon
Wild & Raw EVCLO	3000-5000

Above values calculated from analyses performed at a number of European testing institutes

(named above), and each testing institute analyses has been performed in duplicate.

VITAMIN A₂ (3,4-DEHYDRORETINOL) ANALYSIS

SAMPLE ID	mg/kg
Wild & Raw EVCLO	> = 0.1

> = Greater Than or Equal To.

Above values calculated from analyses performed at a number of European testing institutes

(named above), and each testing institute analyses has been performed in duplicate.

VITAMIN D₃ (CHOLECALCIFEROL) ANALYSIS^#

SAMPLE ID	iu/teaspoon
Wild & Raw EVCLO	400-500

Above values calculated from analyses performed at a number of European testing institutes

(named above), and each testing institute analyses has been performed in duplicate.

^#The vitamin D in Wild & Raw EVCLO is the same form of vitamin D that your body produces when

exposed to sunlight and the only form found in significant quantities in unrefined fish liver oils.

RATIO VITAMINS A:D

SAMPLE ID	ratio
Wild & Raw EVCLO	Typically less than 10:1

STEROL CONTENT

SAMPLE ID	mg/Kg
Wild & Raw EVCLO
CHOLESTEROL	4044-5245
BRASSICASTEROL (PHYTOSTEROL)	40.4
Rest to follow

Above values calculated from analyses performed at a number of European testing institutes

(named above), and each testing institute analyses has been performed in duplicate.

Select Fatty Acid Composition of Total Lipid from Wild & Raw EVCLO

Wild & Raw EVCLO contains a wide spectrum of fatty acids including omega 3, 4, 6, 7, 9, 11 etc.

The test data for selective fatty acids are given below. Levels of other fatty acids not shown are available on request.

Fatty acid	mg/teaspoon
20:5n-3 (EPA)	510
22:6n-3 (DHA)	700
18:1n-9 (Oleic acid)	1,200
20:1n-9 (Gadoleic acid)*	660
22:1n-11 (Cetoleic acid)* Fatty acids, Sum of Omega 6 Calc. Fatty acids, Sum of Omega 3 Calc. Unidentified fatty acids	375 160 1,460 240
	ratio
DHA/EPA^∆	1:1 – 1.3:1
Omega 6/Omega 3 Ratio	0.11

*Gadoleic acid [which was first noted in cod liver oil] and Cetoleic acid are present in higher amounts in codfish from Northern hemisphere and high latitude waters. These fatty acids are therefore found in higher quantities in genuine cod liver oil originating from the Northern hemisphere.

*Cetoleic acid is also usually present in lower proportions than Gadoleic acid.

^∆This ratio is a good indicator of genuine Northern hemisphere cod liver oil.

2. Purity

Cod Liver Oil has been tested for purity by leading European institutes and meets all the stringent European requirements.

HEAVY METAL CONTENT

[ALS Food & Pharmaceutical (UK): 10/10/2012]

	SAMPLE ID
	Wild & Raw EVCLO
MERCURY	n.d
CADMIUM	n.d
LEAD	n.d
ARSENIC	1.4 ppm*

ppm - parts per million

n.d - not detected

*organic (non-toxic) form

HEAVY METAL ANALYSIS

[BioDetection Systems b.v. (BDS): 19/03/2013]

		SAMPLE ID
		Wild & Raw EVCLO
MERCURY		n.d
CADMIUM		n.d
LEAD		n.d
ARSENIC		1.8 ppm*

ppm - parts per million

n.d - not detected (the concentration of the analyte is below the limit of quantification).

*organic (non-toxic) form

ORGANIC CONTAMINANTS [Dioxins and PCBs, Benzo(a)pyrene]

Certificate of Analysis from:

ALS Food & Pharmaceutical (UK) [10/10/2012]
BioDetection Systems b.v. (BDS) [19/03/2013]

Cod liver oil has been analysed in triplicate and each time gives a total WHO-PCDD/F-PCB-TEQ lower than the strict European limits and well within them. The values for the oil are in line with QC parameters.

A. POLYAROMATIC HYDROCARBONS

SAMPLE ID	BENZO(A)PYRENE (mg/kg)
Wild & Raw EVCLO	<0.001^#

^#the concentration of the analyte is below the limit of quantification

Above value calculated from analyses performed in duplicate.

B. DIOXINS and PCBs

Sum of dioxins (WHO-PCDD/F-TEQ): 0.20 ng/kg^#
Sum of dioxins and dioxin-like PCBS (WHO-PCDD/F-PCB-TEQ): 0.899 ng/kg^#

^#Maximum levels set by the European Commission for thesum of dioxins (WHO-PCDD/F-TEQ) for marine oils (fish body oil, fish liver oil and oils of other marine organisms intended for human consumption): 1.75 ng/kg fat

^#Maximum levels set by the European Commission for thesum of dioxins and dioxin like PCBs (WHO-PCDD/F-PCB-TEQ) for marine oils (fish body oil, fish liver oil and oils of other marine organisms intended for human consumption): 6.0 ng/kg fat

^#Dioxins (sum of polychlorinated dibenzo-para-dioxins (PCDDs) and polychlorinated dibenzofurans (PCDFs), expressed as World Health Organisation (WHO) toxic equivalent using the WHO-toxic equivalency factors (WHO-TEFs)) and sum of dioxins and dioxin-like PCBs (sum of PCDDs, PCDFs and polychlorinated biphenyls (PCBs), expressed as WHO toxic equivalent using the WHO-TEFs). WHO-TEFs for human risk assessment based on the conclusions of the World Health Organization (WHO) – International Programme on Chemical Safety (IPCS) expert meeting which was held in Geneva in June 2005 (Martin van den Berg et al., The 2005 World Health Organization Re-evaluation of Human and Mammalian Toxic Equivalency Factors for Dioxins and Dioxin-like Compounds. Toxicological Sciences 93(2), 223–241 (2006))

C. BDS DR CALUX technique: another screening method for dioxins and dioxin-like PCBs

SAMPLE ID	Method	Parameter	Result	Unit
Wild & Raw EVCLO	DR CALUX	PCDD/PCDF and dl-PCBs (total BEQ; semi)	compliant (1.7)	ng BEQ/kg product

The DR CALUX analysis is done according to p-bds-051.

All DR CALUX analysis results comply with EU requirements as indicated in COMMISSION REGULATION (EC) No 278/2012 (amending Regulation (EC) No 152/2009 as regards the determination of the levels of dioxins and polychlorinated biphenyls; feedstuffs).

3. Oxidation byproducts

TOTOX ANALYSIS

Extra virgin oil was also tested by the producer at ECO-Marine using Nofima (the Norwegian Institute of Food, Fisheries and Aquaculture Research) BioLab which specialises in marine raw materials and products [11/11/2013]. Extra virgin oil was also tested as far back as 15.07.2011

SAMPLE ID	PEROXIDE VALUE *	ANISIDINE VALUE *	TOTOX *
Wild & Raw EVCLO	4.8	2.2	11.8

PV expressed as Meq/Kg

AV expressed as anisidine value units

TOTOX = 2PV+AV

TOTOX ANALYSIS

Nutrition Analytical Service, Institute of Aquaculture,

University of Stirling, Scotland, United Kingdom [15/11/2013]

SAMPLE ID	PEROXIDE VALUE *	ANISIDINE VALUE *	TOTOX *
Wild & Raw EVCLO	0.38	0	0.76

PV expressed as Meq/Kg

AV expressed as anisidine value units

TOTOX = 2PV+AV

TOTOX ANALYSIS

Extra virgin oil was also tested by the producer at ECO-Marine using Nofima (the Norwegian Institute of Food, Fisheries and Aquaculture Research) BioLab which specialises in marine raw materials and products [01/02/2014]. Extra virgin oil was also tested as far back as 15.07.2011

SAMPLE ID	PEROXIDE VALUE *	ANISIDINE VALUE *	TOTOX *
Wild & Raw EVCLO	2.9	6.6	12.4

PV expressed as Meq/Kg

AV expressed as anisidine value units

TOTOX = 2PV+AV

*Peroxide value:

NSF limit = < 10 Meq/Kg

CRN limit = 5 Meq/Kg

GOED limit = 5 Meq/Kg

Stringent European Pharmacopoeia Commision max = 5 Meq/Kg

*Anisidine value:

NSF limit = < 20

CRN limit = 20

GOED limit = 20

Stringent European Pharmacopoeia Commision max = 10

*TOTOX:

NSF limit = < 26 Meq/Kg

CRN limit = 26 Meq/Kg

GOED limit = 26 Meq/Kg

Stringent European Pharmacopoeia Commision max = 20 Meq/Kg

NSF: National Sanitation Foundation International

CRN: Council for Responsible Nutrition

GOED: Global Organization for EPA and DHA Omega-3s

TBAR ANALYSIS

Nutrition Analytical Service, Institute of Aquaculture,

University of Stirling, Scotland, United Kingdom [15/11/2013]

SAMPLE ID	TBARS (mg/kg)
Wild & Raw EVCLO	0.25

Above value calculated from analyses performed in duplicate.

TBARs are expressed as mg/kg for feeds and oil and nmole/g tissue for flesh or other tissues.

4. Hydrolytic Rancitiy Measures

[Extra virgin oil was also tested by the producer at ECO-Marine using Nofima (the Norwegian Institute of Food, Fisheries and Aquaculture Research) BioLab which specialises in marine raw materials and products: 01/02/2014]

% FREE FATTY ACID ANALYSIS

SAMPLE ID	%FFA as Oleic
Wild & Raw EVCLO	0.6

ACID VALUE

SAMPLE ID	mg KOH/g
Wild & Raw EVCLO	1.2

5. Microbiological Testing

MICROBIOLOGICAL CONTENT

[ALS Food & Pharmaceutical (UK): 10/10/2012]

SAMPLE ID	cfu/ml
Wild & Raw EVCLO
Total Viable Count	<1
Coliforms (presumptive)	0
Escherichia coli (presumptive)	<1
Yeasts	<2
Moulds	<2
Salmonella sp. (ELISA)	Not detected / 25g

6. Nutrition Facts

Wild & Raw Extra-Virgin Cod Liver Oil: 150 ml

Suggested Serving Size: 1 teaspoon (5 ml) daily

Servings per Bottle: Approximately 30

Amount Per Serving:

Calories 45

Calories from Fat 45

	% Daily Value*
Total Fat 4.5g	7.0%
Saturated Fat 0.9g	4.5%
Trans Fat 0g	0%
Cholesterol 18mg	6%

* Percent Daily Values are based on a caloric intake of 2,000 calories.

Does not contain: Gluten, yeast, milk derivatives, synthetic nutrients, artificial colours or flavours.

7. Shelf-Life Study Results

[Performed by a third party, independent Norwegian laboratory].

Shelf-life of unopened bottles at a temperature of 0-4 °C is 12 months.

For storage at -20 °C the shelf-life is 2-3 times longer.

Shelf-life after the bottles are opened at 0-4 °C is 2-3 months.

Testing has shown that vitamins and other substances found in Rosita EVCLO are not affected by freezing and remain completely intact.

In summary, the oil must be kept dark and cold.

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Extra-Virgin Cod Liver Oil (EVCLO) menu

EVCLO - Nutritional Analysis

1. Nutrient Results