EVCLO - Nutritional Analysis
A wide range of nutritional tests have been performed on Rosita EVCLO. The “Nutritional Analysis” presented represent only a small fraction of this tests which include vitamins, and specialist chemistry testing.
4. Hydrolytic Rancitiy Measures
1. Nutrient Results
The concentration of fat-soluble nutrients depends on several biological factors, including the size of the fish and the amount of oil contained in the liver. There are also seasonal variations related to the spawning cycle of the fish, climatic conditions, plankton, and other conditions affecting the codfish. Because Rosita EVCLO is a completely unrefined & unprocessed wholefood, the concentration of nutrients will ALWAYS vary from batch to batch.
The Nutrient Results (fat-soluble vitamins, sterols & fatty acids) shown below represent typical values and ranges which have been obtained from analytical testing at the following institutes:
- NIFES (National Institute of Nutrition and Seafood Research )[20/12/2010]
- ALS Food & Pharmaceutical (United Kingdom) [10/10/2012]
- Eurofins Food Testing [19/02/2013]
- Nutrition Analytical Service (NAS), Institute of Aquaculture, University of Stirling, Scotland, United Kingdom [15/11/2013]. The head of NAS, Professor Gordon Bell, has over 30 years of experience in fish nutrition and lipid and nutrition related analysis and has published more than 184 peer-reviewed papers on fish nutrition, fatty acids, lipid soluble nutrients and antioxidants. The Chief Analyst, James Dick, provides over 25 years of experience in lipid methodology and is responsible for Quality Control within the Nutrition Group, the development of chromatographic techniques and is trained in all aspects of complex lipid analysis including HPLC, GC, GC/MS and GC/MS/MS.
- Extra virgin oil was also tested [as far back as: 15.07.2011] by the producer at ECO-Marine using a Norwegian Aquaculture Research Institute which specialises in marine raw materials and products.
VITAMIN E (ALPHA-TOCOPHEROL) ANALYSIS
SAMPLE ID |
mg/teaspoon |
Wild & Raw EVCLO |
3.7-10 |
VITAMIN A (RETINOL) ANALYSIS
SAMPLE ID |
iu/teaspoon |
Wild & Raw EVCLO |
3000-5000 |
VITAMIN A2 (3,4-DEHYDRORETINOL) ANALYSIS
SAMPLE ID |
mg/kg |
Wild & Raw EVCLO |
> = 0.1 |
VITAMIN D3 (CHOLECALCIFEROL) ANALYSIS#
SAMPLE ID |
iu/teaspoon |
Wild & Raw EVCLO |
400-500 |
RATIO VITAMINS A:D
SAMPLE ID |
ratio |
Wild & Raw EVCLO |
Typically less than 10:1 |
STEROL CONTENT
SAMPLE ID |
mg/Kg |
Wild & Raw EVCLO |
|
CHOLESTEROL |
4044-5245 |
BRASSICASTEROL (PHYTOSTEROL) |
40.4 |
Rest to follow |
Select Fatty Acid Composition of Total Lipid from Wild & Raw EVCLO
Fatty acid |
mg/teaspoon |
20:5n-3 (EPA) |
510 |
22:6n-3 (DHA) |
700 |
18:1n-9 (Oleic acid) |
1,200 |
20:1n-9 (Gadoleic acid)* |
660 |
22:1n-11 (Cetoleic acid)* Fatty acids, Sum of Omega 6 Calc. Fatty acids, Sum of Omega 3 Calc. Unidentified fatty acids |
375 160 1,460 240 |
ratio |
|
DHA/EPA∆ |
1:1 – 1.3:1 |
Omega 6/Omega 3 Ratio |
0.11 |
∆This ratio is a good indicator of genuine Northern hemisphere cod liver oil.
Cod Liver Oil has been tested for purity by leading European institutes and meets all the stringent European requirements.
HEAVY METAL CONTENT
[ALS Food & Pharmaceutical (UK): 10/10/2012]
SAMPLE ID |
|
Wild & Raw EVCLO |
|
MERCURY |
n.d |
CADMIUM |
n.d |
LEAD |
n.d |
ARSENIC |
1.4 ppm* |
ppm - parts per million
n.d - not detected
*organic (non-toxic) form
HEAVY METAL ANALYSIS
[BioDetection Systems b.v. (BDS): 19/03/2013]
SAMPLE ID |
||
Wild & Raw EVCLO |
||
MERCURY |
n.d |
|
CADMIUM |
n.d |
|
LEAD |
n.d |
|
ARSENIC |
1.8 ppm* |
ppm - parts per million
n.d - not detected (the concentration of the analyte is below the limit of quantification).
*organic (non-toxic) form
ORGANIC CONTAMINANTS [Dioxins and PCBs, Benzo(a)pyrene]
Certificate of Analysis from:
- ALS Food & Pharmaceutical (UK) [10/10/2012]
- BioDetection Systems b.v. (BDS) [19/03/2013]
Cod liver oil has been analysed in triplicate and each time gives a total WHO-PCDD/F-PCB-TEQ lower than the strict European limits and well within them. The values for the oil are in line with QC parameters.
A. POLYAROMATIC HYDROCARBONS
SAMPLE ID |
BENZO(A)PYRENE
(mg/kg)
|
Wild & Raw EVCLO |
<0.001# |
#the concentration of the analyte is below the limit of quantification
Above value calculated from analyses performed in duplicate.
B. DIOXINS and PCBs
- Sum of dioxins (WHO-PCDD/F-TEQ): 0.20 ng/kg#
- Sum of dioxins and dioxin-like PCBS (WHO-PCDD/F-PCB-TEQ): 0.899 ng/kg#
#Maximum levels set by the European Commission for thesum of dioxins (WHO-PCDD/F-TEQ) for marine oils (fish body oil, fish liver oil and oils of other marine organisms intended for human consumption): 1.75 ng/kg fat
#Maximum levels set by the European Commission for thesum of dioxins and dioxin like PCBs (WHO-PCDD/F-PCB-TEQ) for marine oils (fish body oil, fish liver oil and oils of other marine organisms intended for human consumption): 6.0 ng/kg fat
#Dioxins (sum of polychlorinated dibenzo-para-dioxins (PCDDs) and polychlorinated dibenzofurans (PCDFs), expressed as World Health Organisation (WHO) toxic equivalent using the WHO-toxic equivalency factors (WHO-TEFs)) and sum of dioxins and dioxin-like PCBs (sum of PCDDs, PCDFs and polychlorinated biphenyls (PCBs), expressed as WHO toxic equivalent using the WHO-TEFs). WHO-TEFs for human risk assessment based on the conclusions of the World Health Organization (WHO) – International Programme on Chemical Safety (IPCS) expert meeting which was held in Geneva in June 2005 (Martin van den Berg et al., The 2005 World Health Organization Re-evaluation of Human and Mammalian Toxic Equivalency Factors for Dioxins and Dioxin-like Compounds. Toxicological Sciences 93(2), 223–241 (2006))
C. BDS DR CALUX technique: another screening method for dioxins and dioxin-like PCBs
SAMPLE ID |
Method |
Parameter |
Result |
Unit |
Wild & Raw EVCLO |
DR CALUX |
PCDD/PCDF and dl-PCBs (total BEQ; semi) |
compliant (1.7) |
ng BEQ/kg product |
The DR CALUX analysis is done according to p-bds-051.
All DR CALUX analysis results comply with EU requirements as indicated in COMMISSION REGULATION (EC) No 278/2012 (amending Regulation (EC) No 152/2009 as regards the determination of the levels of dioxins and polychlorinated biphenyls; feedstuffs).
TOTOX ANALYSIS
Extra virgin oil was also tested by the producer at ECO-Marine using a Norwegian Aquaculture Research Institute which specialises in marine raw materials and products. Extra virgin oil was also tested as far back as 15.07.2011
SAMPLE ID |
PEROXIDE VALUE * |
ANISIDINE VALUE * |
TOTOX * |
Wild & Raw EVCLO |
4.8 |
2.2 |
11.8 |
PV expressed as Meq/Kg
AV expressed as anisidine value units
TOTOX = 2PV+AV
TOTOX ANALYSIS
SAMPLE ID |
PEROXIDE VALUE * |
ANISIDINE VALUE * |
TOTOX * |
Wild & Raw EVCLO |
0.38 |
0 |
0.76 |
PV expressed as Meq/Kg
AV expressed as anisidine value units
TOTOX = 2PV+AV
TOTOX ANALYSIS
Extra virgin oil was also tested by the producer at ECO-Marine using a Norwegian Aquaculture Research Institute which specialises in marine raw materials and products. Extra virgin oil was also tested as far back as 15.07.2011
SAMPLE ID |
PEROXIDE VALUE * |
ANISIDINE VALUE * |
TOTOX * |
Wild & Raw EVCLO |
2.9 |
6.6 |
12.4 |
PV expressed as Meq/Kg
AV expressed as anisidine value units
TOTOX = 2PV+AV
TBAR ANALYSIS
SAMPLE ID |
TBARS (mg/kg) |
Wild & Raw EVCLO |
0.25 |
Above value calculated from analyses performed in duplicate.
TBARs are expressed as mg/kg for feeds and oil and nmole/g tissue for flesh or other tissues.
4. Hydrolytic Rancitiy Measures
[Extra virgin oil was also tested by the producer at ECO-Marine using a Norwegian Aquaculture Research Institute which specialises in marine raw materials and products. Extra virgin oil was also tested as far back as 15.07.2011]
SAMPLE ID |
%FFA as Oleic |
Wild & Raw EVCLO |
0.6 |
SAMPLE ID |
mg KOH/g |
Wild & Raw EVCLO |
1.2 |
MICROBIOLOGICAL CONTENT
[ALS Food & Pharmaceutical (UK): 10/10/2012]
SAMPLE ID |
cfu/ml |
Wild & Raw EVCLO |
|
Total Viable Count |
<1 |
Coliforms (presumptive) |
0 |
Escherichia coli (presumptive) |
<1 |
Yeasts |
<2 |
Moulds |
<2 |
Salmonella sp. (ELISA) |
Not detected / 25g |
Calories 45
|
Calories from Fat 45
|
|
% Daily Value*
|
Total Fat 4.5g
|
7.0%
|
Saturated Fat 0.9g
|
4.5%
|
Trans Fat 0g
|
0%
|
Cholesterol 18mg
|
6%
|
[Performed by a third party, independent Norwegian laboratory].
- Shelf-life of unopened bottles at a temperature of 0-4 °C is 12 months.
- For storage at -20 °C the shelf-life is 2-3 times longer.
- Shelf-life after the bottles are opened at 0-4 °C is 2-3 months.
- Testing has shown that vitamins and other substances found in Rosita EVCLO are not affected by freezing and remain completely intact.
- In summary, the oil must be kept dark and cold.